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e coli strain t7express  (New England Biolabs)


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    New England Biolabs e coli strain t7express
    E Coli Strain T7express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t7+express+competent+e%2E+coli/pm42119206-543-7-11?v=New+England+Biolabs
    Average 97 stars, based on 1319 article reviews
    e coli strain t7express - by Bioz Stars, 2026-07
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    Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.

    Journal: Nucleic Acids Research

    Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens

    doi: 10.1093/nar/gkag338

    Figure Lengend Snippet: Detection of E. coli rRNA modifications by LC-MS/MS following Tth-MazF1 Cleavage. ( A ) A schematic of a Tth-MazF1 digest of a mixture of E. coli rRNA isolated from 70S ribosomes. ( B ) Fractional sequence coverage of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. ( C ) Sequence coverage maps of detected high-confidence cleavage products in Tth-MazF1 digests of E. coli rRNA. A complete list and description of the modifications are provided in . Data are a summary of two replicate experiments.

    Article Snippet: Candidate genes were cloned into expression vectors as described in the section “semi-high-throughput expression and purification of maltose-binding protein (MBP)-MazF-6×His proteins.” T7 Express competent E. coli cells (NEB, C1013I) were transformed with 10 ng of each plasmid and plated on LB agar supplemented with kanamycin (50 μg/ml).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation, Sequencing